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ISSN: 1557-4555 (Print)
ISSN: 1557-4563 (Online)
Research Article Open Access

Detection of tet Gene in Multidrug-Resistant Salmonella spp. Isolates from Layers and Broiler Breeders

Agustin Indrawati1, Leila Nur Azizah1, I Wayan Teguh Wibawan1, Usamah Afiff1, Safika1, Maya Shofa1 and Siti Gusti Ningrum2
  • 1 IPB University, Bogor, Indonesia
  • 2 Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia

Abstract

The present study was conducted with the following aims: (i) To detect the multidrug-resistant Salmonella spp. isolates recovered from faeces, litter and drinking water in layers and broiler breeders’ farms and (ii) to carried out the detection of the tet gene. A total of 21 Salmonella isolates were subjected to Polymerase Chain Reaction (PCR) assay to determine the presence of tet gene. Out of 21 isolates, 14 (66.7%) and 7 (33.3%) were found positive for tet(A) and tet(B), respectively. In antimicrobial susceptibility tests, the Salmonella isolates showed resistance to tetracycline, oxytetracycline, ampicillin, nalidixic acid, enrofloxacin, gentamicin and chloramphenicol. It can be concluded that the high prevalence of the tet gene indicates a high potential of Salmonella isolates for horizontal transmission of tetracycline resistance genes.

American Journal of Animal and Veterinary Sciences
Volume 15 No. 2, 2020, 163-168

DOI: https://doi.org/10.3844/ajavsp.2020.163.168

Submitted On: 23 April 2020 Published On: 17 June 2020

How to Cite: Indrawati, A., Azizah, L. N., Wibawan, I. W. T., Afiff, U., Safika, ., Shofa, M. & Ningrum, S. G. (2020). Detection of tet Gene in Multidrug-Resistant Salmonella spp. Isolates from Layers and Broiler Breeders. American Journal of Animal and Veterinary Sciences, 15(2), 163-168. https://doi.org/10.3844/ajavsp.2020.163.168

Open AccessCopyright: © 2020 Agustin Indrawati, Leila Nur Azizah, I Wayan Teguh Wibawan, Usamah Afiff, Safika, Maya Shofa and Siti Gusti Ningrum. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Multidrug-Resistant
  • Salmonella
  • tet(A)
  • tet(B)