Research Article Open Access

A common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case Study

Jyoti Bhardwaj1 and Sudesh Kumar Yadav1
  • 1 Biotechnology Division, CSIR-IHBT, Palampur-176061 (HP), India

Abstract

Three protocols viz. Trichloroacetic Acid (TCA)-acetone, phenol and multi-detergent were compared for the extraction of proteins from horsegram (Macrotyloma uniflorum) which is less studied yet nutritionally and economically very important pulse crop. Almost equal quantity of proteins was extracted by all the three protocols. However, quality of protein from phenol method was superior to that from other protocols. Bands in One-Dimensional (1-D) and Two-Dimensional Electrophoresis (2-DE) of phenol protocol were reproducible and better. We report here a modified and common protocol for the extraction of proteins for proteomic analysis. The developed protocol is applicable not only to other economically important leguminous but also non-leguminous crops.

American Journal of Agricultural and Biological Sciences
Volume 8 No. 4, 2013, 293-301

DOI: https://doi.org/10.3844/ajabssp.2013.293.301

Submitted On: 25 January 2012 Published On: 28 October 2013

How to Cite: Bhardwaj, J. & Yadav, S. K. (2013). A common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case Study. American Journal of Agricultural and Biological Sciences, 8(4), 293-301. https://doi.org/10.3844/ajabssp.2013.293.301

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Keywords

  • Trichloroacetic Acid (TCA)
  • Pinch of Bromophenol Blue (PBB)
  • Two-Dimensional Electrophoresis (2-DE)
  • One-Dimensional (1-D)
  • Sodium Dodecyl Sufate (SDS)