TY  - JOUR
AU  - Bhardwaj, Jyoti 
AU  - Yadav, Sudesh Kumar 
PY  - 2013
TI  - A common Protein Extraction Protocol for Proteomic Analysis: Horse Gram a Case Study
JF  - American Journal of Agricultural and Biological Sciences
VL  - 8
IS  - 4
DO  - 10.3844/ajabssp.2013.293.301
UR  - https://thescipub.com/abstract/ajabssp.2013.293.301
AB  - Three protocols viz. Trichloroacetic Acid (TCA)-acetone, phenol and multi-detergent were compared for the extraction of proteins from horsegram (Macrotyloma uniflorum) which is less studied yet nutritionally and economically very important pulse crop. Almost equal quantity of proteins was extracted by all the three protocols. However, quality of protein from phenol method was superior to that from other protocols. Bands in One-Dimensional (1-D) and Two-Dimensional Electrophoresis (2-DE) of phenol protocol were reproducible and better. We report here a modified and common protocol for the extraction of proteins for proteomic analysis. The developed protocol is applicable not only to other economically important leguminous but also non-leguminous crops.