Research Notes Open Access

Functionalization Strategies for Antibodies: Lessons Learned

Christian Schmidt1, Martin Geyer1, Jörg Bohrisch1, Verena Jentzen1, Alexander Gorczyza1, Karim Khalil1, Marina Volkert1, Mark A. Brown2, Saadettin Sel3 and Joachim Storsberg1
  • 1 Fraunhofer-Institute for Applied Polymer Research (IAP), Germany
  • 2 Colorado State University, United States
  • 3 Heidelberg University, Germany

Abstract

Precious little is known about useful functionalization strategies of antibodies that will not impair either reactivity or specificity of this valuable reagent. We used an experimental approach to demonstrate our own experience with published protocols. The conjugates obtained were then tested with regard to their performance against commercially available detection kits. Our results are then viewed in light of published precedence to highlight areas where future effort is needed to refine such versatile tools.

American Journal of Immunology
Volume 13 No. 2, 2017, 144-154

DOI: https://doi.org/10.3844/ajisp.2017.144.154

Submitted On: 10 April 2017 Published On: 4 May 2017

How to Cite: Schmidt, C., Geyer, M., Bohrisch, J., Jentzen, V., Gorczyza, A., Khalil, K., Volkert, M., Brown, M. A., Sel, S. & Storsberg, J. (2017). Functionalization Strategies for Antibodies: Lessons Learned. American Journal of Immunology, 13(2), 144-154. https://doi.org/10.3844/ajisp.2017.144.154

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Keywords

  • Chemistry
  • Immunology
  • Antibody
  • Reactivity
  • Specificity