Research Article Open Access

Nitric Oxide Donors with Therapeutic Strategic in Experimental Schistossomiasis Mansoni

Wander Rogério Pavanelli1, Jean Jerley Nogueira da Silva2, Carolina Panis1, Thiago Mattar Cunha3, Francisco José de Abreu Oliveira1, Maria Claudia Noronha Dutra de Menezes1, Ivete Conchon Costa1, Graciele da Silva Thomé1, Francisco Ordelei Nascimento da Silva4, Eduardo Henrique Silva de Sousa4, Luiz Gonzaga de França Lopes4, Rubens Cecchini1, Fernando de Queiroz Cunha3, Eiko Nakagawa Itano1 and Maria Angélica EharaWatanabe1
  • 1 State University of Londrina-UEL, Brazil
  • 2 State University of Roraima, Brazil
  • 3 Faculty Medicine of Ribeirão Preto-USP, Brazil
  • 4 Federal University of Ceará, Brazil


Schistosomiasis, an immune disease, remains a major public health problem in endemic area. To determine the influence of Nitric Oxide (NO) on this disease, we tested two compounds (Trans-[Ru(bpy)2(NO)SO3](PF6)-PF6 and Na2[Fe(CN)5(NO)]-SNP, which releases NO when activated by biological reducing agents, in BALB/c mice infected subcutaneously by Schistosoma BH strains. The parasitic activity of NO-donors was evaluated in this model by measuring the immune cellular response in liver with: Cytokines levels; histopathological characteristics and the number of the granulomatous lesions; and NO levels. We found that NO-donors treated mice were more resistant to infection, since they exhibited higher survival. Furthermore, we observed in histopathological analysis a decreased influx of inflammatory cells in the hepatic tissue of mice treated with both donors. The parasite counting (estimated as eggs and worms number) was also minor in treated mice. Moreover, decreased levels of IL-10 were detected in the liver of infected mice treated with SNP. The animals treated with PF6 showed high plasmatic NO levels at 45 days after infection. Altogether, these data suggest that NO is a pivotal factor of resistance during schistossomiasis by controlling parasites proliferation, influencing cytokine production and consequently modulating the development of inflammatory response.

American Journal of Immunology
Volume 10 No. 4, 2014, 225-239


Submitted On: 27 March 2014 Published On: 24 February 2015

How to Cite: Pavanelli, W. R., da Silva, J. J. N., Panis, C., Cunha, T. M., Oliveira, F. J. A., Menezes, M. C. N. D. D., Costa, I. C., Thomé, G. D. S., Silva, F. O. N. D., Sousa, E. H. S. D., Lopes, L. G. F., Cecchini, R., Cunha, F. Q., Itano, E. N. & EharaWatanabe, M. A. (2014). Nitric Oxide Donors with Therapeutic Strategic in Experimental Schistossomiasis Mansoni. American Journal of Immunology, 10(4), 225-239.

  • 5 Citations



  • NO Donors
  • PF6
  • SNP
  • Nitric Oxide
  • Immune Response
  • Schistossomiasis