Research Article Open Access

Isolation and Mutagenesis of Streptokinase Producing Bacteria

Thaer Taleb Abed Abdelghani1, Adinarayana Kunamneni1 and Poluri Ellaiah1
  • 1 Department of Pharmaceutical Sciences Andhra University, India


Of 103 β-haemolytic Streptococci (with haemolytic activity) in total were isolated from different samples of blood and biomass from infected throat, 47 isolates exhibited streptokinase (SK) activity. T3, identified as a new variant of Streptococcus equisimilis, was found to be excellent SK producer in submerged fermentation and was selected for strain improvement studies. This strain was mutagenized by UV and N-methyl-N'-nitro-N-nitroso guanidine (NTG). The isolation of mutants and the SK activity of selected mutants were described. The SK yield of the best UV mutant AUV10 was 120% higher than the wild strain (T3). Also, the SK yield of the best NTG mutant NUV7 was 146% higher than the wild strain (T3). The results indicated that UV and NTG were effective mutagenic agents for strain improvement of S. equisimilis T3 for enhanced SK productivity.

American Journal of Immunology
Volume 1 No. 4, 2005, 125-129


Submitted On: 9 March 2006 Published On: 31 December 2005

How to Cite: Abdelghani, T. T. A., Kunamneni, A. & Ellaiah, P. (2005). Isolation and Mutagenesis of Streptokinase Producing Bacteria. American Journal of Immunology, 1(4), 125-129.

  • 10 Citations



  • Streptokinase (SK)
  • screening
  • isolation
  • mutagenesis
  • bacteria