Research Article Open Access

Optimization of Preparation Procedures of Phlorizin-Zn (Ⅱ) Complex and Its Antioxidant Activity Study in vitro

Yuqing Zhang1, Yaping Li2, Guorui Yang2, Shaoxuan Yu2, Haifang Xiao2 and Yuanda Song2
  • 1 Department of Food Science and Engineering, Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zibo, Shandong, China
  • 2 Department of Food Science and Engineering, Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zibo, Shandong, China

Abstract

Flavonoid metal complexes possess more effective antioxidant properties than flavonoids. However, the preparation of the phlorizin-Zn (Ⅱ) complex and its antioxidant activity has not been reported. In this research, the preparation procedures of the phlorizin-Zn (Ⅱ) complex were enhanced using by single factor test and Response Surface Methodology (RSM). The optimal preparation procedures of the phlorizin-Zn (II) complex were as follows: The time of 4.1 h, the pH of 5.9, the temperature of 48.7°C and the molar ratio (phlorizin: Zn2+) of 1:1. Under these conditions, the predicted preparation yield of phlorizin-Zn (Ⅱ) complex was 79.052%. Additionally, the antioxidant activity of the phlorizin-Zn (Ⅱ) complex was analyzed in this research. 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH), 2, 2'-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic acid) (ABTS), hydroxyl free radical and Ferric ion Reducing Antioxidant Power (FRAP) experiments revealed that phlorizin-Zn (Ⅱ) complex had significant DPPH, ABTS and hydroxyl free radical scavenging abilities and exhibited substantial reducing power in a dose-dependent manner. Moreover, the phlorizin-Zn (Ⅱ) complex displayed stronger in vitro antioxidant capacity compared with phlorizin. The findings demonstrated that the phlorizin-Zn (Ⅱ) complex had great potential in antioxidant applications in the future.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 4, 2023, 373-383

DOI: https://doi.org/10.3844/ajbbsp.2023.373.383

Submitted On: 15 November 2023 Published On: 3 April 2024

How to Cite: Zhang, Y., Li, Y., Yang, G., Yu, S., Xiao, H. & Song, Y. (2023). Optimization of Preparation Procedures of Phlorizin-Zn (Ⅱ) Complex and Its Antioxidant Activity Study in vitro. American Journal of Biochemistry and Biotechnology, 19(4), 373-383. https://doi.org/10.3844/ajbbsp.2023.373.383

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Keywords

  • Phlorizin-Zn (Ⅱ) Complex
  • Preparation Procedure
  • Response Surface Methodology
  • Antioxidant Activity