Effect of Adding Cellulose on Lipid Accumulation in Cellobiohydrolase and Delta-6 Desaturase Engineered Mucor circinelloides Strains
- 1 Department of Food Science and Nutrition, Food Bioengineering and Technology Laboratory, College of Culture and Tourism, University of Jinan, 13 Shungeng Road, Jinan, China
- 2 Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zibo, China
Abstract
Despite microbial production of multifunctional gamma-linolenic acid (GLA) being an important and promising way, the high cost is still a major factor limiting industrial applications. Previously, we proposed a novel approach to promote the production of GLA from cellulose, a widely available and cheapest resource, by overexpression of the cellobiohydrolase (CBH2) and delta-6 desaturase (D6) in Mucor circinelloides. In the present study, an in-depth analysis of growth and lipid accumulation in engineered M. circinelloides strains using cellulosic substrate was to be explored. When cultivated in modified Kendrick and Ratledge (K and R) medium supplemented with the ratio of 1: 1 for glucose and carboxymethyl cellulose (CMC), the cellobiohydrolase and delta-6 desaturase overexpressing strains led to increases in the biomass (up to 10.4 g/L) and lipid yield (up to 2.6 g/L) of 68% and 2.2-fold, respectively, compared with that of the control strain. The yield of GLA in the cellobiohydrolase and delta-6 desaturase co-expression strains reached 415 mg/L, which was a remarkable increase of 3.1-fold compared to that of the control strain. These results proved that the introduction of CBH2 and D6 two key enzymes could significantly enhance the utilization of CMC and effectively convert cellulose into GLA by engineered M. circinelloides. This study provided a reference for the further potential application of the engineered M. circinelloides strains in the conversion of functional GLA from cellulose.
DOI: https://doi.org/10.3844/ajbbsp.2023.275.281
Copyright: © 2023 Yao Zhang, Yanxia Wang, Qing Liu and Yuanda Song. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Γ-Linolenic Acid
- Mucor circinelloides
- Cellulose
- Cellobiohydrolase
- Delta-6 Desaturase