Research Article Open Access

Effect of Mercuric Ion on the Oxidant Response and Calcium Ion in the Protoplasts of Arabidopsis Thaliana

Jun Xing1, Rui Wu1, Hui Yu1, Cailing Yuan1 and Xue Yang1
  • 1 Drug Research and Development Center, Shandong Drug and Food Vocational College, Weihai 264200, China

Abstract

As a non-essential element for plant growth and development, the heavy metal mercury (Hg) has significant toxicity to plants. The aim of this study was to investigate the effect of mercuric ion (Hg2+) on the oxidant response and calcium ion (Ca2+) in the protoplasts of Arabidopsis thaliana. The protoplast cells were exposed to 5.0, 10.0, and 20.0 mmol/L Hg2+ for 0.5 and 1.0 h, respectively. To detect the effect of Hg2+ on the Reactive Oxygen Species (ROS) with the laser scanning confocal microscopy, 2’ 7’-Dichlorofluorescent yellow Diacetate (DCFH-DA) was used to label ROS in protoplast cells of Arabidopsis thaliana. The result of confocal microscopy analysis showed that the level of ROS was increased in protoplast cells after the treatments of 5.0, 10.0, and 20.0 mmol/L Hg2+. However, the activity of antioxidant enzymes Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GSH-PX) in protoplasts was decreased by 5.0, 10.0, and 20.0 mmol/L Hg2+ treatments. In addition, ion chromatography was used to study the effect of Hg2+ on the content of Ca2+ in the protoplasts of Arabidopsis thaliana. It showed that the content of Ca2+ in the outer cell of protoplasts was increased after the treatments of 5.0, 10.0, and 20.0 mmol/L Hg2+. In conclusion, Hg2+ decreases the activity of antioxidant enzymes and induces the elevation of ROS in the protoplast cells. Mercuric ion inhibits the absorption of Ca2+ by protoplast cells and the change of Ca2+ content may affect ROS level in the protoplasts of Arabidopsis thaliana.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 3, 2023, 227-236

DOI: https://doi.org/10.3844/ajbbsp.2023.227.236

Submitted On: 8 April 2023 Published On: 29 August 2023

How to Cite: Xing, J., Wu, R., Yu, H., Yuan, C. & Yang, X. (2023). Effect of Mercuric Ion on the Oxidant Response and Calcium Ion in the Protoplasts of Arabidopsis Thaliana. American Journal of Biochemistry and Biotechnology, 19(3), 227-236. https://doi.org/10.3844/ajbbsp.2023.227.236

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Keywords

  • Mercury
  • Protoplasts
  • Reactive Oxygen Species
  • Calcium Ions
  • Arabidopsis Thaliana