Research Article Open Access

Epidemiology and Detection of Bacterial Diarrhea Using Multiplex Polymerase Chain Reaction, Focusing on Campylobacter spp.

Eun Ju Oh1, Tae Su Jang2, Jung Min Park3 and Jae Kyung Kim4
  • 1 Department of Medical Laser, Dankook University Graduate School of Medicine, Republic of Korea
  • 2 Department of Health Administration, Dankook University College of Health and Welfare, Republic of Korea
  • 3 Department of Public Health Science, Dankook University Graduate School of Natural Science, Republic of Korea
  • 4 Department of Biomedical Laboratory Science, Dankook University College of Health Sciences, Korea

Abstract

This study assessed the clinical characteristics, epidemiological trends, and changes associated with major pathogens causing gastrointestinal inflammation. We retrospectively analyzed the test results of hospitalized patients who were administered a mPCR test for diarrhea causing bacteria between June 2010 and December 2019 at the Cheonan Dankook University hospital in the Republic of Korea and identified clinical characteristics and epidemiological trends related to major pathogens causing gastrointestinal inflammation. Our results showed that infections due to Clostridium perfringens, Escherichia coli H7, Campylobacter species (spp.), and Clostridioides difficile toxin B varied seasonally (March to October). When examining annual trends, we found that Escherichia coli H7 incidence decreased significantly throughout the time frame examined, from 27.27% of total infections in 2010 to 8.93% in 2019. In contrast, the incidence of both Clostridium spp., and Campylobacter spp. gradually increased throughout this time frame. Our study was able to demonstrate that improvements in environmental hygiene have aided in reducing the occurrence of water borne diarrhea, while infections due to food borne, anaerobic and diarrhea causing bacteria, such as those present in non-sterile milk and poultry, are increasing. We believe that this study will serve as a useful resource for better understanding the dynamics and risks associated with bacterial diarrhea, including infections caused by Campylobacter spp.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 1, 2023, 121-127

DOI: https://doi.org/10.3844/ajbbsp.2023.121.127

Submitted On: 29 December 2022 Published On: 27 March 2023

How to Cite: Oh, E. J., Jang, T. S., Park, J. M. & Kim, J. K. (2023). Epidemiology and Detection of Bacterial Diarrhea Using Multiplex Polymerase Chain Reaction, Focusing on Campylobacter spp.. American Journal of Biochemistry and Biotechnology, 19(1), 121-127. https://doi.org/10.3844/ajbbsp.2023.121.127

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Keywords

  • Acute Diarrhea
  • Campylobacter
  • Clostridoides Difficile
  • Clostridium perfringens
  • Escherichia coli H7