Research Article Open Access

ELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) Species

Phumzile Mkhize1 and Phetole Mangena1
  • 1 University of Limpopo, South Africa

Abstract

Fusarium contains the most toxigenic and important fungal pathogens, producing a wide range of mycotoxins that include the more toxic secondary metabolites called fumonisins. This study analyzed the presence of common exo- and mycelium-soluble antigens, purified proteins using reducing SDS-PAGE and the expression of antibodies against different fungal pathogens. The results showed the presence of common antigens between pathogens and development of antibodies against antigens from F. circinatum, F. oxysporum and D. pinea. A strong reactivity against the antigens ranged between 0.510 to 0.868 in F. circinatum and F. oxysporum isolates. The reactivity of anti-D. pinea antibodies was relatively low, with the highest absorbance value of 0.650, accompanied by a minimal cross reactivity. Overall, these findings indicated that, antibodies against mycelium-soluble antigens were the most immuno-reactive and could be helpful for the development of a rapid Fusarium detection tool for use in various industries.

American Journal of Biochemistry and Biotechnology
Volume 16 No. 4, 2020, 529-537

DOI: https://doi.org/10.3844/ajbbsp.2020.529.537

Submitted On: 23 August 2020 Published On: 18 November 2020

How to Cite: Mkhize, P. & Mangena, P. (2020). ELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) Species. American Journal of Biochemistry and Biotechnology, 16(4), 529-537. https://doi.org/10.3844/ajbbsp.2020.529.537

  • 2,448 Views
  • 928 Downloads
  • 0 Citations

Download

Keywords

  • Antibodies
  • Antigens
  • ELISA
  • Fusarium
  • SDS-PAGE
  • Western Blotting