Research Article Open Access

Effect of Ultra-High Pressure Technology on Isomerization and Antioxidant Activity of Lycopene in Solanum Lycopersicum

Siqun Jing1, Zainixi Lasheng2, Saisai Wang2 and Hui Shi2
  • 1 Shaoguan University, Daxue Road 288, Zhenjiang District, Shaoguan, Guangdong 512005, China
  • 2 Xinjiang University, Shengli Road 14, Urumqi, Xinjiang 830046, China

Abstract

To evaluate the influence of the Ultra-High Pressure processing (UHP) on the isomerization and antioxidant activity of lycopene in tomatoes (Solanum lycopersicum), High Performance Liquid Chromatography (HPLC) and Ultraviolet-Visible Spectroscopy (UV-Vis) were used to separate and identify the isomer of lycopene. The scavenging activities against DPPH∙and∙OH present samples with and without UHP treatment were tested with ascorbic acid as positive control. Full wavelength scanning figure showed that a characteristic absorption peak was created at 361 nm, which was caused by trans-lycopene isomerizing into cis isomer. HPLC analysis found that five peaks (Rt = 19.082, 13.307, 9.790, 8.123, 7.207 min) were produced after UHP treatment, which were further identified by UV-Vis and four peaks among them showed a strong absorption peak at 361 nm which was in accordance with the UV-Vis spectroscopy of cis-lycopene. The total antioxidant, capable of scavenging hydroxyl radicals·OH and DPPH·radical of lycopene treated by UHP, were all higher than that of control and lycopene without UHP treated. Taken together, UHP treatment played the role in cis-trans conversion of lycopene and could also improve the antioxidant activity of lycopene oleoresin with a dose-dependent fashion.

American Journal of Biochemistry and Biotechnology
Volume 16 No. 2, 2020, 270-279

DOI: https://doi.org/10.3844/ajbbsp.2020.270.279

Submitted On: 4 March 2019 Published On: 21 June 2020

How to Cite: Jing, S., Lasheng, Z., Wang, S. & Shi, H. (2020). Effect of Ultra-High Pressure Technology on Isomerization and Antioxidant Activity of Lycopene in Solanum Lycopersicum. American Journal of Biochemistry and Biotechnology, 16(2), 270-279. https://doi.org/10.3844/ajbbsp.2020.270.279

  • 2,867 Views
  • 1,148 Downloads
  • 0 Citations

Download

Keywords

  • Ultra-High Pressure Processing
  • Lycopene
  • Isomerization
  • Antioxidant Activity