cDNA Cloning and Characterization of the House Dust Mite Allergen Sui p 2
- 1 University of Santo Tomas, Philippines
Abstract
Suidasia pontifica (Sp) is a House Dust Mite (HDM) species found in tropical urban residential areas which causes sensitization of up to 75% of allergic patients. Cloning of allergens from Sp is a significant initial step in understanding the clinical significance of this HDM species. The study describes the cloning and in silico characterization of the group 2 allergen gene from Sp (Sui p 2) with significant homology to Myeloid Differentiation 2 (MD-2) lipid-binding (ML domain) protein allergens. Sui p 2 gene was amplified by Reverse Transcription – PCR using gene-specific primers designed from known HDM group 2 allergens. The amplicons were characterized using NCBI BLAST, ExPASy, MEGA7 and Chimera. Sui p 2 cDNA gene exhibits percent identity to Tyrophagus putrescentiae (65.84%), Aleuroglyphus ovatus (65.69%) and Blomia tropicalis (62.98%). Sui p 2 gene contains 405 bp open reading frame with untranslated regions on its 5’ and 3’ end. Furthermore, structure-based percent identity of Sui p 2 to the group 2 allergen of D. farinae and D. pteronyssinus is 47.62% and 46.83%, respectively. Predicted protein structure reveals a putative immunoglobulin fold consisting of preserved cysteine residues, hydrophobic core and two antiparallel β-sheets containing several TLR4, LPS and IgE binding sites. The results suggest that Sui p 2 may play an important role in HDM induced allergy and may be involved in the induction of the innate immune system. Characterization of Sui p 2 is important to evaluate properly its use for allergy component resolved diagnosis and allergen-specific immunotherapy.
DOI: https://doi.org/10.3844/ajbbsp.2020.222.234
Copyright: © 2020 Joshua Evans M. Bajao and John Donnie A. Ramos. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Allergen
- House Dust Mite
- Innate Immunity
- T-cell Epitope
- Toll-Like Receptors