Research Article Open Access

Optimization of Cellulase-Assisted Extraction of Total Flavonoids from Corn Bract and Evaluation of Antioxidant and Antibacterial Aactivities

Yang Zhang1, Jing Lu2, Leijie Ben1, Lixue Zheng1, Pengfei Xu1, Yingang Jia1, Zhenyu Cheng2 and Zhaowei Yan3
  • 1 Changshu Institute of Technology, China
  • 2 Jilin Institute of Chemical Technology, China
  • 3 The First Affiliated Hospital of Soochow University, China

Abstract

Corn bract is a corn-based agricultural waste material that distributed worldwide. In present work, response surface methodology was applied to optimize the cellulase-assisted extraction of Total Flavonoids from Corn Bract (TFCB), the in vitro antioxidant and antibacterial as well as the in vivo antioxidant activities of TFCB were investigated. Results showed that the optimal conditions for TFCB extraction were as follows: Amount of cellulase, 0.4% (w/w); incubation time, 2 h; liquid-to-solid ratio, 35: 1 mL/g; ethanol concentration, 71% (v/v); TFCB yield was 1.284±0.01%, which was 25.39% higher than that of heat reflux extraction. In addition, when compared with vitamin C, TFCB showed weaker in vitro free radical-scavenging capacities, but stronger antioxidant activities in mice. Moreover, TFCB also exerted certain inhibitory effects against Gram-positive bacteria. This study will provide an evidence for the potential of comprehensive utilization of discarded corn bract.

American Journal of Biochemistry and Biotechnology
Volume 15 No. 2, 2019, 61-74

DOI: https://doi.org/10.3844/ajbbsp.2019.61.74

Submitted On: 20 January 2019 Published On: 18 May 2019

How to Cite: Zhang, Y., Lu, J., Ben, L., Zheng, L., Xu, P., Jia, Y., Cheng, Z. & Yan, Z. (2019). Optimization of Cellulase-Assisted Extraction of Total Flavonoids from Corn Bract and Evaluation of Antioxidant and Antibacterial Aactivities. American Journal of Biochemistry and Biotechnology, 15(2), 61-74. https://doi.org/10.3844/ajbbsp.2019.61.74

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Keywords

  • Corn Bract
  • Total Flavonoids
  • Cellulase-Assisted Extraction
  • Antioxidant Activity
  • Antibacterial Activity