Research Article Open Access

Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression

Hidekatsu Yanai1, Hiroshi Yoshida2, Hironobu Fujiwara1, Shigeru Yoshida1 and Hirotoshi Fuda3
  • 1 Hokkaido University School of Medicine, Japan
  • 2 The Jikei University School of Medicine, Japan
  • 3 The Health Sciences University of Hokkaido Ishikari-Tobetsu, Japan

Abstract

Oxidized low-density lipoprotein (LDL) has been shown to be a powerful regulator of gene expression in monocyte-derived macrophage. To determine the effects of oxidized LDL on macrophage gene expression, macrophages incubated with native or oxidized LDL were analyzed by differential display technique. The differentially expressed cDNA (387 bp) fragment by oxidized LDL showed 100% homology to the part of 3’-untranslated region of the gene of human GM2 activator protein. Quantitative RT-PCR data using specific TaqMan probe showed the increased GM2 activator protein mRNA expression in macrophages incubated with oxidized LDL by about twice (215.4±31.6 %) of that in macrophages incubated with native LDL. Our results shows that ox-LDL upregulates GM2 activator protein gene expression, indicating that GM2 activator protein may be associated with the pathogenesis of atherosclerosis.

American Journal of Biochemistry and Biotechnology
Volume 1 No. 2, 2005, 90-94

DOI: https://doi.org/10.3844/ajbbsp.2005.90.94

Submitted On: 7 June 2005 Published On: 30 June 2005

How to Cite: Yanai, H., Yoshida, H., Fujiwara, H., Yoshida, S. & Fuda, H. (2005). Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression. American Journal of Biochemistry and Biotechnology, 1(2), 90-94. https://doi.org/10.3844/ajbbsp.2005.90.94

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Keywords

  • Oxidized LDL
  • macrophage
  • GM2 activator protein
  • differential display