Research Article Open Access

A New Method to Estimate Intrinsic Parameters in the Ping-pong Bisustrate Kinetic: Application to the Oxipolymerization of Phenol

José L. Gómez1, Antonio Bódalo1, Elisa Gómez1, Asunción M. Hidalgo1 and María Gómez1
  • 1 University of Murcia, Spain

Abstract

A new method for determining the intrinsic parameters of reaction in processes involving a high initial rate has been developed. The usefulness of this alternative, which consists of determining several sets of apparent parameters at different times and then extrapolating these to time zero, is demonstrated proved by the linear dependence obtained between the apparent parameters and the reaction time. The method permitted the values of the intrinsic parameters (enzyme specific activity and Michaelis-Menten constants of both substrates) to be obtained for the system under study and was checked with experimental reaction rate data for the soybean peroxidase/phenol/hydrogen peroxide system.

American Journal of Biochemistry and Biotechnology
Volume 1 No. 2, 2005, 115-120

DOI: https://doi.org/10.3844/ajbbsp.2005.115.120

Submitted On: 13 May 2005 Published On: 30 June 2005

How to Cite: Gómez, J. L., Bódalo, A., Gómez, E., Hidalgo, A. M. & Gómez, M. (2005). A New Method to Estimate Intrinsic Parameters in the Ping-pong Bisustrate Kinetic: Application to the Oxipolymerization of Phenol. American Journal of Biochemistry and Biotechnology, 1(2), 115-120. https://doi.org/10.3844/ajbbsp.2005.115.120

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Keywords

  • Bisubstrate kinetic
  • intrinsic parameters
  • soybean peroxidase enzyme
  • phenol
  • hydrogen peroxide
  • wastewater treatment