Research Article Open Access

Enzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose Hydrolysis

Nur Izyan Wan Azelee1, Jamaliah Md Jahim2, Ahmad Fauzi Ismail3, Siti Fatimah Zaharah Mohamad Fuzi4, Roshanida A. Rahman1, Nazlee Faisal Ghazali1 and Rosli Md Illias1
  • 1 Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia
  • 2 Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
  • 3 Advanced Membrane Technology Research Centre, Universiti Teknologi Malaysia, 81310, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia
  • 4 Department of Technology and Heritage, Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia, 86400, Parit Raja, Batu Pahat, Johor Darul Takzim, Malaysia

Abstract

An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield.

American Journal of Agricultural and Biological Sciences
Volume 11 No. 2, 2016, 54-66

DOI: https://doi.org/10.3844/ajabssp.2016.54.66

Submitted On: 29 June 2015 Published On: 28 April 2016

How to Cite: Azelee, N. I. W., Jahim, J. M., Ismail, A. F., Fuzi, S. F. Z. M., Rahman, R. A., Ghazali, N. F. & Illias, R. M. (2016). Enzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose Hydrolysis. American Journal of Agricultural and Biological Sciences, 11(2), 54-66. https://doi.org/10.3844/ajabssp.2016.54.66

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Keywords

  • Enzymatic Hydrolysis
  • Hemicellulose
  • Kenaf
  • Reaction Conditions
  • Xylanase