Research Article Open Access

Attenuated Total Reflectance-Fourier-Transform Infrared Microspectroscopy a Rapid Method for Microbial Strain Characterization

Meshref Awad Alruwaili1
  • 1 College of Applied Medical Science, Aljouf University, Clinical Lab. Science, B.O. 2014, Skaka, Saudi Arabia

Abstract

Fourier-Transform Infrared (FTIR) spectroscopy and BHI 50CH were used to identify bacteria of the Lactobacillus (L.) at the species level. A previously developed method for measuring FTIR spectra and a strategy for their analysis provided the basis for selecting the FTIR spectra of reference bacterial strains and created a spectral library. The research was launched in which the spectra collected in the above library were used for developing a spectral reference for known reference bacterial strains and the practical value of the generated library was verified based on the results of identification of four bacterial strains viz. L. plantarum, L. casei, L. lactis and L. fermentum of known taxonomy as well as identification of 15 bacterial strains isolated from rumen extracts and identified on the basis of their taxonomy and biochemical tests. The application of prepared lactic acid bacteria reference library for analysis of advanced analysis of FTIR was provided an accurate identification of 90% of bacterial strains of the genus Lactic acid bacteria identified by FTIR-microspectroscopy.

American Journal of Agricultural and Biological Sciences
Volume 8 No. 2, 2013, 135-141

DOI: https://doi.org/10.3844/ajabssp.2013.135.141

Submitted On: 1 May 2013 Published On: 8 June 2013

How to Cite: Alruwaili, M. A. (2013). Attenuated Total Reflectance-Fourier-Transform Infrared Microspectroscopy a Rapid Method for Microbial Strain Characterization. American Journal of Agricultural and Biological Sciences, 8(2), 135-141. https://doi.org/10.3844/ajabssp.2013.135.141

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Keywords

  • Lactic Acid Bacteria
  • FTIR Spectra
  • Rumen Extract
  • Bacterial Isolation