@article {10.3844/ajbbsp.2022.339.348, article_type = {journal}, title = {Preparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina Velutipes}, author = {Peng, Yingyun and Jiang, Han and Wu, Yufeng and Zhang, Jinlong and Zhou, Jianhua and Chen, Yiyong}, volume = {18}, number = {3}, year = {2022}, month = {Sep}, pages = {339-348}, doi = {10.3844/ajbbsp.2022.339.348}, url = {https://thescipub.com/abstract/ajbbsp.2022.339.348}, abstract = {In this study, a novel polysaccharide FVP2a wasextracted and purified from F. velutipes. Ac-FVP2a was prepared bychemical acetylation. The preparation process of Ac-FVP2a was optimized usingthe response surface method based on three experimental factors includingreaction time, reaction temperature, and the ratio of FVP2a to aceticanhydride. The physicochemical characteristics andantioxidant activities of Ac-FVP2a in vitro were investigated. The results showed that the optimalpreparation process of Ac-FVP2a was a reaction time of 2.5 h, reactiontemperature of 45℃ and ratio of FVP2ato acetic anhydride of 1:37 (g/mL), respectively. The monosaccharides of Ac-FVP2awith an average molecular weight of 1.97 × 105 Da were galactose,glucose, rhamnose, xylose, and arabinose and the molar ratio were1.65:1:0.13:0.26:1.12. FT-IR suggested that Ac-FVP2a was prepared successfully.Ac-FVP2a had stronger antioxidant activities with better thermal stability thanFVP2a. Ac-FVP2a might have the potential to become a novel antioxidant in thefood industry.}, journal = {American Journal of Biochemistry and Biotechnology}, publisher = {Science Publications} }