@article {10.3844/ajbbsp.2022.118.128,
article_type = {journal},
title = {Radiation Triggered Phenotypic Changes of Regulatory T Cells},
author = {Liu, Lantao and Peng, Yan and Zhang, Deqin and Niu, Dongsheng},
volume = {18},
number = {1},
year = {2022},
month = {Mar},
pages = {118-128},
doi = {10.3844/ajbbsp.2022.118.128},
url = {https://thescipub.com/abstract/ajbbsp.2022.118.128},
abstract = {Ionizing radiation disrupts the immune balance easily. Regulatory T (Treg) cells directly affect the immune balance after radiation exposure. Meanwhile, the present study is to investigate the survival ability and phenotypic changes of Treg cells induced by 60Co γ-rays in order to expand the understanding on how Treg cells modulated by radiation. Mice lymphocytes were isolated from thymus and spleen at 1, 4 and 10 days after 2 Gy 60Co γ-rays irradiation. The proliferation and phenotype of Treg cells were analyzed by flow cytometry. Helio+ Treg cells were calculated and ATP luminescence was measured by ELISA. CD39 from thymus and spleen was measured by flow cytometry. Moreover, miR-31 expression in the thymus and spleen post irradiation was determined by RT-PCR. The apoptosis rate of Treg cells was increased to 13.1% in thymus and 44.2% in the spleen after 2Gy irradiation. The proliferation of Treg cells in thymus decreased from 31.6 to 14.2% post irradiation (t = 3.56, 5.72, P},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}