@article {10.3844/ajbbsp.2021.433.447,
article_type = {journal},
title = {Transcriptomic Analysis of the Erythritol High-Yielding Mutant Strain Yarrowia lipolytica Y44},
author = {Ren, Xiaojie and Ban, Heng and Huang, Chao and Liu, Baoyue and Song, Yuanda and Zhao, Xinhe and Pei, Jiangsen},
volume = {17},
number = {4},
year = {2021},
month = {Dec},
pages = {433-447},
doi = {10.3844/ajbbsp.2021.433.447},
url = {https://thescipub.com/abstract/ajbbsp.2021.433.447},
abstract = {RNA-seq technique was used to analyze the transcriptomics of Yarrowia lipolytica control strain Po1g, an erythritol-producing natural isolate Yarrowia lipolytica Y 22 and an erythritol high-yielding mutant strain Y44 derived from Y 22. Functional annotation of genes and classification of metabolic pathways were carried out to identify Differentially Expressed Genes (DEGs). These DEGs were classified into related metabolic pathways to explain the molecular mechanism of high yield of erythritol production. The results showed that the upregulated genes in erythritol-producing natural isolate Y 22 and erythritol-producing mutant strain Y 44 were mainly involved in pentose phosphate pathway, Tricarboxylic Acid Cycle (TCA) and malic acid cycle. The downregulated genes were mainly involved in amino acid synthesis and oxycarboxylic acid metabolism. The synergistic regulation of the above metabolic pathways can increase the input and reduce the output of erythrose-4-P (E-4-P), which is the precursor of erythritol, promoting erythritol production. In addition, compared with the natural isolate Y 22, the genes related to cell wall synthesis were down-regulated and the expression of transmembrane transporter protein was up-regulated in high-yield mutant strain Y 44. In this way, the permeability of yeast cells is enhanced and the synthesized erythritol can be quickly transported to the outside of the cell, which reduces the decomposition of erythritol and further promotes its yield.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}