TY  - JOUR
AU  - Ubonbal, Raksmont 
AU  - Posoongnoen, Saijai 
AU  - Daduang, Jureerut 
AU  - Klaynongsruang, Sompong 
AU  - Daduang, Sakda 
PY  - 2015
TI  - Amino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)
JF  - American Journal of Biochemistry and Biotechnology
VL  - 11
IS  - 3
DO  - 10.3844/ajbbsp.2015.119.126
UR  - https://thescipub.com/abstract/ajbbsp.2015.119.126
AB  - The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid residues for the active site. It was classified as a member of α-amylase family 13 because it has an active Domain A, similar to a (α/β)8-barrel structure. Three-dimensional structural predictions revealed that this partial sequence completely covered all of necessary domains for amylase activity.