TY  - JOUR
AU  - Ahmed, Aimun Abdelgaffar Elhassan 
AU  - Gaspar, Robert 
AU  - Marki, Arpad 
AU  - Vasas, Andrea 
AU  - Mudawi, Mahmoud Mudawi Eltahir 
AU  - Hohmann, Judit 
AU  - Falkay, George 
PY  - 2010
TI  - Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)
JF  - American Journal of Biochemistry and Biotechnology
VL  - 6
IS  - 3
DO  - 10.3844/ajbbsp.2010.231.238
UR  - https://thescipub.com/abstract/ajbbsp.2010.231.238
AB  - Problem statement: The aim of this study is to investigate the pharmacological effects of the Methanolic-extract (AH2) of El-Hazha and its sub-fractions. Approach: These investigations were carried out on in vitro isolated uterus preparations from Non-Pregnant (NP) and Late-Pregnant rats (LP). In parallel displacement radio-ligand binding assay was performed for β-Adrenergic Receptors (β-ADR). Results: Showed that the herb and its different fractions produced dose-dependent relaxant effect (p<0.05, t-test, n = 6) on uterine contraction elicit by 25 mM KCl in both NP and LP uteri that affected significantly by fractionation, however, the effect of the most active fraction (AH2-11) was reversed by prior addition of propranolol (non-specific β-antagonist), but not affected by progesterone pre treatment of the LP rats. In addition, AH2 only in high concentration displaced isotopes from β-ADR, this affinity changed markedly by fractionation. Conclusion: We validate the fractionation effect on its relaxant activity and found partial role for β-ADR on mediating this activity. Future study was recommended to isolate and investigate its active components to enhance this activity or to discover a new novel natural therapeutic agent(s).