TY  - JOUR
AU  - Alagendran, S. 
AU  - Archunan, G. 
AU  - Rameshkumar, K. 
AU  - Kadalmani, B. 
AU  - Rodriguez, Jorge Arturo León 
AU  - Fernandez, Gabriela 
AU  - Martinez, G. 
AU  - Guzman, Rosalinda Guevara 
PY  - 2010
TI  - 2-Nonenal-Ovulatory Specific Volatiles in Human Saliva throughout Menstrual Cycle by Gas Chromatography and Mass Spectrometry Analysis
JF  - American Journal of Biochemistry and Biotechnology
VL  - 6
IS  - 3
DO  - 10.3844/ajbbsp.2010.187.194
UR  - https://thescipub.com/abstract/ajbbsp.2010.187.194
AB  - Problem statement: The present investigation carry out a pilot study of a novel method to
identify the salivary volatiles in different phases of menstrual cycle for the assessment of ovulation
detection using gas chromatography and mass spectrophotometer. Approach: The profiles from
follicular phase (6-12 days); ovulatory phase (13-14 days) and luteal phase (15-26 days) of menstrual
cycle samples were compared to establish any qualitative and quantitative differences that might have
potential value in human olfactory communication. Dichloromethane was used as the solvent for
extraction of the compounds. Results: Fifteen compounds were identified. They include organic
compounds like, acid, aldehyde, amine and alcohol. The most important constituent was 2-nonenal,
which usually comprised 75% or more of the total volatiles observed in ovulatory phase. The
concentration of many constituents varied widely. This appeared to be periodically in three cycles for
five of the constituents, with a period of a few weeks and with pronounced maxima at the peak of
ovulatory period of which only two were common to all the chromatograms. The chemical profile of
ovulatory phase saliva was distinguished by the presence of two specific compounds, viz. 2-
Nonenal, Acetic acid and Acetaldehyde that were not found in the other reproductive phases of
saliva sample in women. Apparently these compounds are 2-nonenal, dodecanol, acetic acid and
acetaldehyde. One or more of these compounds may have pheromonal activity in human body odor.
Conclusion: Differentiation of the volatile patterns among reproductive phases in women may help to
find the diagnostic marker for ovulation detection.