TY  - JOUR
AU  - Salleh, Mohd. Nazil 
AU  - Shi, Boo Siaw 
AU  - Ramli, Wan Khairuzzaman Wan 
AU  - Zahari, Azlina 
AU  - Shamsudin, Norashikin 
AU  - Hashim, Thuaibah 
PY  - 2010
TI  - Detection and Localization of Anti and Pro-apoptotic mRNA Genes in Human Colorectal Cancer Using in situ RT-PCR
JF  - American Journal of Biochemistry and Biotechnology
VL  - 6
IS  - 3
DO  - 10.3844/ajbbsp.2010.164.171
UR  - https://thescipub.com/abstract/ajbbsp.2010.164.171
AB  - Problem statement: Recent studies using conventional Polymerase Chain Reaction have
shown that anti-apoptotic (Cyclooxygenase-2, COX-2 and Nuclear Factor-κB, NF-κB) and proapoptotic
mRNA (Bax and Bad) are involved in the tumorigenesis of colorectal cancer. The aim of this
study was to localize the expression of anti and pro-apoptotic mRNA genes using Reverse
Transcription in situ Polymerase Chain Reaction (RT in situ PCR) and immunodetection technique in
the early stage of human colorectal adenocarcinoma. Approach: Reverse Transcription in situ
Polymerase Chain Reaction (RT in situ PCR) and immunodetection technique was applied throughout
of this studies. 20 paraffin-embedded tissue blocks of human colorectal adenocarcinoma samples was
used compared to controls. Results: Morphologically, the glands and crypts were well-differentiated,
enlarged and irregular with active secretion of mucin. COX-2, NF-κB, Bax and Bad mRNA were
expressed in both normal and human colorectal cancer tissues. All mRNA genes were expressed in the
cytoplasm and nuclei. However, COX-2 and NF-κB mRNA genes were highly expressed with higher
intensity of brown staining compared to Bax and Bad at tubular epithelium cells. Conclusion: This
study demonstrated that by using RT in situ PCR, COX-2 and NF-κB mRNA genes were shown to be
involved in the development of human colorectal cancer.