TY  - JOUR
AU  - Norouzian, D. 
AU  - Akbarzadeh, A. 
AU  - Abnosi, M.H. 
AU  - Atyabi, S.M. 
AU  - Farahngi, A. 
AU  - Mehrabi, M.R. 
AU  - Eslamifar, A. 
PY  - 2007
TI  - Stabilization of Tyrosinase-Bovine Serum Albumin Crystals by Glutaraldehyde
JF  - American Journal of Biochemistry and Biotechnology
VL  - 3
IS  - 3
DO  - 10.3844/ajbbsp.2007.110.113
UR  - https://thescipub.com/abstract/ajbbsp.2007.110.113
AB  - Tyrosinase and bovine serum albumin were co-crystallized by saturated ammonium sulfate solution(65%) and 20% polyethylene glycol ( PEG) 6000 and n-propanol as co-solvents .The obtained crystals were cross linked by glutaraldehyde solution(1% v/v).Polyethylene glycol 6000 was found to be better co-solvent than n-propanol. The developed biocatalyst could be recycled 6 times without further loss of tyrosinase activity. No loss of activity of cross linked tyrosinase -bovine serum albumin crystals was observed upon storage of the developed CLECs at refrigerator for six months.