TY  - JOUR
AU  - Ehsanpour, Ali Akbar 
AU  - Razavizadeh, Roya 
PY  - 2005
TI  - Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus
JF  - American Journal of Biochemistry and Biotechnology
VL  - 1
IS  - 2
DO  - 10.3844/ajbbsp.2005.107.110
UR  - https://thescipub.com/abstract/ajbbsp.2005.107.110
AB  - Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0, 15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20, 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.