@article {10.3844/ajavsp.2010.71.75,
article_type = {journal},
title = {Effect of Equilibration Temperature on In vitro Viability and Subsequent Embryo Development of Vitrified-Warmed Immature Bovine Oocytes},
author = {Hadi, Hajarian and Wahid, H. and Mazni, O. Abas and Rosnina, Y. and Daliri, M. and Dashtizad, M. and Faizah, A. and Yap, K.C. and Fahrul, F.J. and Fazly, A.},
volume = {5},
number = {2},
year = {2010},
month = {Sep},
pages = {71-75},
doi = {10.3844/ajavsp.2010.71.75},
url = {https://thescipub.com/abstract/ajavsp.2010.71.75},
abstract = {Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equilibration temperatures on in vitro viability of immature bovine oocytes after vitrification. Approach: Oocytes were obtained from slaughterhouse ovaries. Only grade one oocytes were used. Oocytes were equilibrated in three different temperatures: 32, 37, or 41°C. Immature oocytes were equilibrated in VS1 (7.5 Ethylene Glycol (EG) + 7.5% DMSO) for 10-12 min and then exposed to VS2 (15% EG + 15%DMSO + 0.5M sucrose) for one min. Thereafter oocytes were loaded on hand-made Cryotop and directly plunged into liquid nitrogen. After warming, oocytes were examined for viability, maturation, cleavage and blastocyst production. Results: Oocytes that were equilibrated at 37°C had significantly higher (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}