TY - JOUR AU - Dashtizad, Mojtaba AU - Haron, Abd Wahid AU - Yusoff, Rosnina AU - Daliri, Morteza AU - Hajarian, Hadi AU - Najari, Mehdi AU - Chee, Yap Keng AU - Othman, Abas Mazni PY - 2010 TI - Synergistic Effect of Insulin on in vitro Development of Immature Bovine Oocytes JF - American Journal of Animal and Veterinary Sciences VL - 5 IS - 4 DO - 10.3844/ajavsp.2010.258.265 UR - https://thescipub.com/abstract/ajavsp.2010.258.265 AB - Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes and in vitro fertilized embryos. Approach: Grade one cumulus-oocyte-complexes harvested from slaughterhouse ovaries were selected and randomly allocated in each treatment groups. In experiment 1, in vitro maturation medium (Hepes-buffered medium 199 + fetal calf serum + gonadotrophins + antibiotics) supplemented with 0 (control), 1, 10, 20 and 100 μg mL-1 of insulin. In experiment 2, to eliminate the effect of serum and hormones, Hepes-buffered medium 199 was supplemented with 1 mg mL-1 polyvinyl alcohols (PVA) and same levels of insulin. In experiment 3, the effect of insulin on bovine in vitro embryo development was assessed. Presumptive zygotes were randomly cultured in synthetic oviductal fluid added with 0 (control), 1, 10, 20 and 100 μg mL-1 of insulin. Results: In experiment 1, nuclear maturation and embryo development rates were significantly higher in 1 and 10 μg mL-1 compared with other groups (P