@article {10.3844/ajabssp.2009.79.82,
article_type = {journal},
title = {Development of Microbial Inoculants and the Impact of Soil Application on Rice Seedlings Growth},
author = {Al-Taweil, Hayyan Ismaeil and Osman, Mohammad Bin and Hamid, Aidil Abdul and Wan Yussof, Wan Mohtar},
volume = {4},
year = {2009},
month = {Mar},
pages = {79-82},
doi = {10.3844/ajabssp.2009.79.82},
url = {https://thescipub.com/abstract/ajabssp.2009.79.82},
abstract = {Problem statement: The present study was undertaken to assess 
the Empty Fruit Bunches (EFB) as carrier of Biofertilizer and microbial inoculants 
interaction. A study in Malaysia had shown a strain of Trichoderma viride and 
Bacillus megaterium were isolated from the soil. Questions were raised whether 
the tow inoculants compatible to be mixed in same carrier? It is important to 
develop delivery system of the inoculants. The present study was undertaken to 
assess the Empty Fruit Bunches (EFB) as carrier of biofertilizers and microbial 
inoculants interaction and the impact on rice seedlings. Approach: 
Bacillus negaterium and Trichoderma viride were grown in their respective broth 
culture. Cells numbers in broth pr suspension were determined. EFB powder 200 
g in polypropylene bags was sterilized. Inoculums from broth of suspension were 
added in EFB using sterilized syringe, moisture level in each packet was maintained 
by adding respective broth, 1 g was periodically withdraw from fomenters, soaked 
in 10 mL sterilized distal water, then serially diluted and plated out, the number 
of viable cells was expressed as cfu g-1 dry root. Twenty one-day-old rice seedlings 
of MR220 cultivar were grown under greenhouse conditions in autoclaved homogeneous 
soil, rice seedlings were treated with Trichoderma and Bacillus. Five replicates 
were used for each treatment. Rice seedlings height (cm), root length (cm), root 
numbers, root and shoot dray matter, parameters were measured during and after 
experiment. All data were analyzed at an alpha level of 0.05 or 0.10 using experimental 
design by completely randomized design model using the analysis of variance module 
(treatments × 3 R) all treatment means were separated using Fisher’s protected 
least significant difference (LSD) mean separation. Results: 
Trichoderma growth in carrier reduces Bacillus growth as interaction effect of
both in same carrier which indicated that Bacillus unable to develop on multi-inoculants biofertilizers
with Trichoderma. Inoculation of rice seedlings with Bacillus megaterium and Trichoderma viride
produced maximum shoots and roots dry matter, increased significantly root length numbers and
seedlings height comparing with control and same action of NPK fertilizers. Conclusion: 
The present study showed the possibility to develop the delivery system of biofertilizers 
which contained Bacillus and Trichoderma as mono inoculums not as multi-inoculants 
due to incompatible of both in same carrier. Bacillus and Trichoderma also showed 
appositive effect with rice seedlings.},
journal = {American Journal of Agricultural and Biological Sciences},
publisher = {Science Publications}
}